Use of 8-azaguanine for the isolation of auxotrophic mutants of Bacillus megaterium.
نویسندگان
چکیده
Wachsman, J. T. (University of Illinois, Urbana) and R. Mangalo. Use of 8-azaguanine for the isolation of auxotrophic mutants of Bacillus megaterium. J. Bacteriol. 83:35-37. 1962.-8-Azaguanine is bactericidal for Bacillus megaterium strain KM, primarily under conditions where growth is possible. This analogue has been used to enrich for auxotrophic mutants in ultraviolet-irradiated populations. The survivors were found to contain from 1.6 to 33.0% amino acid auxotrophs and from 19 to 29% pyrimidine nucleoside auxotrophs. Since many different biological systems are sensitive to 8-azaguanine, the use of this analogue to enrich for auxotrophic mutants may have a more general application than techniques currently employed.
منابع مشابه
Use of 5-fluorouracil for the isolation of auxotrophic mutants of Bacillus megaterium.
Wachsman, J. T. (University of Illinois, Urbana), and L. Hogg. Use of 5-fluorouracil for the isolation of auxotrophic mutants of Bacillus megaterium. J. Bacteriol. 87:1137-1139. 1964.-The combination of 5-fluorouracil (FU) and uridine was used to selectively kill wild-type cells of Bacillus megaterium KM, thereby providing surviving populations greatly enriched in auxotrophic mutants. Exponenti...
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عنوان ژورنال:
- Journal of bacteriology
دوره 83 شماره
صفحات -
تاریخ انتشار 1962